The present study was conducted to record the incidence, clinical presentation, electrocardiographic, radiographic, laboratory, two dimensional echocardiography, M-mode echocardiography, pulsed wave Doppler and color flow Doppler findings in Labrador Retrievers with Dilated Cardiomyopathy (DCM) for a period of five years from 2013 to 2018. It included 210 healthy dogs and 327 confirmed cases of DCM. The incidence of dilated cardiomyopathy in Labrador Retrievers was found to be 7.49 per cent in the present study. On radiography, cardiomegaly and pulmonary edema were the major findings observed. In echocardiography, increased left ventricular end diastolic dimension and systolic dimension, reduced fraction shortening, increased E-point sepal separation, increased Left atrium (LA) / Aorta (AO) ratio, decreased ejection fraction, increased end diastolic volume and end systolic volume were noticed. On pulsed wave Doppler echocardiography reduced pulmonary artery (PA), Aorta (AO), left ventricular outflow tract (LVOT), right ventricular outflow tract (RVOT) velocities were recorded. Mild to moderate regurgitation was observed in Mitral and Tricuspid valve by color flow Doppler echocardiography. M-mode derived chamber dimensions, E-point septal separation, ejection fraction, fractional shortening were reliable parameters in diagnosing Dilated Cardiomyopathy in Labrador Retrievers. Pulsed wave Doppler and color flow Doppler were useful in assessing velocity and flow pattern across valves.