Year: 2016 | Month: April | Volume 6 | Issue 2

Identification of Immunoreactive Polypeptides in Haemonchus contortus by Immunoprecipitation during Prepatent Infection in Sh

DOI:Coming soon...


In the current study immunoreactive polypeptides in Adult Somatic Extract (ASE), Excretory Secretory Product (ESP) and Larval Somatic Extract (LSE) of L3 stage were identified by employing immunoprecipitation, a lesser utilized technique on helminth parasites. Prepatent polyclonal sera were used to capture polypeptides in immunoprecipitation. Polypeptides eluted from the immune complex in ASE in SDS-PAGE were 26, 60, 120 & 170 kDa whereas in ESP these were 26, 32, 60 and 120 kDa showing absence of 170 kDa and presence of 32 kDa in ESP. In LSE the polypeptides captured were 26, 32, 60 & 120 kDa. Possible functional significance of the peptides in different antigens has been discussed and development of an immunoprophylactic agent has been speculated.

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Print This Article Email This Article to Your Friend

@ Journal of Animal Research | In Association with Association of Mastitis

19581455 - Visitors since March 23, 2019