Year: 2017 | Month: February | Volume 7 | Issue 1

Isolation, Morphological Identification and In vitro Antibacterial Activity of Endophytic Bacteria Isolated From Morus nigra (Mulberry) L


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Abstract:

Plant-associated bacteria that live inside plant tissues without causing any harm to plants are defined as endophytic bacteria. Different parts of mulberry plant (root, stem and leaves) are reported to possess different pharmacological activity.The present study was done to isolate endophytic bacteria from Morus nigra (mulberry) leaves, their identification and investigate their antibacterial activity against three gram positive bacteria, Staphylococcus aureus, Streptococcus pyogens and Bacillus cereus and gram negative bacteria Escherichia coli, Salmonella Typhimurium and Klebsiella pneumoniae. A total 25 leaves samples were taken, they were sterilized with 0.1 per cent sodium hypochlorite, 0.01 per cent bavistin, 0.05 per cent and 70 per cent ethanol. Sterilized leaves of the plants were embedded in kings B petri plates. for the isolation of endophytic bacteria. Maximum isolated sample on kings b media were irregular in shape, flat elevation, undulated margin, glistening growth surface, opaque and white in colour, the microscopic examination revealed that isolated endophytic bacteria were gram positive and rod shaped. The antibacterial effect was studied by the disc diffusion method with known antibiotic ciprofloxacin (Ci) as standard. The antibacterial activity of endophytic bacteria isolated from Morus nigra (mulberry) showed good antibacterial activity against Streptococcus pyogens.



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