Year: 2017 | Month: February | Volume 7 | Issue 1
Standardization of Protocols for Extraction of Aloe vera and Cinnamon Bark Extract
The present study was envisaged to optimize the extraction protocols viz. time, temperature and solvent concentration for extraction of phyto-extracts from aloe vera and cinnamon bark based on antioxidant efficacy in-vitro. These were optimized using Box-Behnken Design (BBD) and completely randomized design (CRD) of Response Surface Methodology (RSM). A total number of 17 different combinations of time, temperature and ethanol concentration in BBD and 13 different combinations of time, temperature in CRD were considered for optimizing extraction protocols for aloe vera and cinnamon respectively. The in-vitro 1, 1 diphenyl-2picrylhydrazyl (DPPH % inhibition) and 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) ABTS+ radical scavengering activity of aqueous extract of cinnamon bark was recorded highest for the combination of 99% concentration at 80°C temperature for 3h amongst all 13 runs. The 90% ethanolic extract of aloe vera and cinnamon bark exhibited higher in-vitro antioxidant ability than aqueous extract at time, temperature combination of 15 min for 65°C and 9h for 60°C respectively.
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