Year: 2021 | Month: April | Volume 11 | Issue 2

Altered Immune Response to Decellularized Porcine Small Intestinal Sub-Mucosa (DPSIS) Graft in Dogs Suffering from Keratoconjunctivitis Sicca

Sowbharenya Chelladurai Kiranjeet Singh Aswathy Gopinathan Ravi Kant Agrawal Pawan Kumar Naveen Kumar Monalisa Sahoo
DOI:10.30954/2277-940X.02.2021.2

Abstract:

Study was conducted to explore immune response to decellularised porcine small intestinal sub-mucosa (DPSIS) graft though clinical evaluation and mRNA expression of selected genes of ocular surface epithelium after corneal surface reconstruction in dogs suffering from Keratoconjunctivitis sicca (KCS). Sixteen client-owned dogs that underwent deep lamellar keratoplasty (DALK) and DPSIS graft for corneal surface reconstruction were selected; seven dogs with corneal ulcer were included in group A as control and nine dogs with corneal ulcer as a complication of KCS were included in group B as treatment group. Healing of corneal ulcer under graft was evaluated clinically and by digital photographs. Gene expression of ICAM-1, VCAM-1, PD-L1 and FASL before and after grafting was done by real-time polymerase chain reaction (qRT-PCR) at definite intervals post graft. DPSIS graft promoted effective healing of corneal defect of partial/full thickness in group A and preserved corneal integrity whereas fibrosis and corneal pigmentation ensued in group B post DPSIS graft. Gene expression of ICAM-1 and VCAM-1 were found elevated in group B before and after PSIS grafting. PD-L1 mRNA expression was significantly increased in group B. Protein and gene expression of ICAM-1 and VCAM-1 varied with severity of inflammation in corneal surface post DPSIS grafting. Enhanced mRNA expression of PD- L1 and inflammatory mediators ICAM-1 and VCAM-1 in the ocular surface epithelial cells suggested an altered immune response to DPSIS graft and warranted strategies to modify them for successful repair of corneal epithelial defects in KCS affected dogs.

Highlights

  • Ocular surface epithelial cells of dogs with KCS showed increased gene expression of PD-L1, ICAM-1, and VCAM-1.
  • Fate of DPSIS graft in cornea of KCS affected dogs is influenced by its altered immune status.


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