Year: 2021 | Month: April | Volume 11 | Issue 2

Detection and Molecular Characterization of Avian Infectious Laryngotracheitis Virus Isolated from a Breeder Flock

S. Jaisree K. Shoba A. Ramesh K. Vijayarani S. Vairamuthu R. Bharathi
DOI:10.30954/2277-940X.02.2021.5

Abstract:

Infectious laryngotracheitis (ILT) is an economically important viral respiratory disease in poultry. Recently, re-emergence of Infectious laryngotracheitis virus (ILTV) has been reported in several countries. This study reports the severe form of ILTV outbreak in a breeder flock located at Chittoor district of Andhra Pradesh. Morbidity and mortality rate observed in the current outbreak were 70% and 12% respectively. On post mortem examination haemorrhage and caseous plug were noticed in the trachea. PCR for ICP4 gene was targeted for initial screening and ILTV was isolated from positive samples. Molecular characterization was carried out by gJ gene sequencing, one of the virulent gene responsible for viral egress. Phylogenetic analysis revealed that the study isolate is genetically related to Australian virulent virus, Italy virulent virus, Russian virulent virus, USA virulent virus and CEO vaccines.

Highlights

  • This paper reports the outbreak of avian infectious laryngotracheitis in a breeder flock at Chittoor.
  • Diagnosis was carried out targeting ICP4 gene of ILTV and molecular characterization was carried out with gJ gene.
  • Phylogenetic analysis revealed that the study isolate was related to Australian virulent virus, Italy virulent virus, etc.


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