Year: 2022 | Month: February | Volume 12 | Issue 1

In vitro Anticancer Activity of Silver Nanoparticles Biosynthesized from Seeds of Sesamum indicum Against Dalton’s Lymphoma Ascites

V. Keerthika A.R. Nisha S. Sujith R. Shankar Arya Mohan P. Gowtham
DOI:10.30954/2277-940X.01.2022.4

Abstract:

The objective of this study was to explore the anticancer activity of silver nanoparticles green synthesized from aqueous extract of Sesamum indicum (S. indicum) seeds in Dalton’s lymphoma ascitic cells. The anticancer efficacy of biosynthesized silver nanoparticles from plant extract (S-AgNPs) was compared relatively with chemically synthesized nanoparticles (C-AgNPs), which were produced using trisodium citrate. The C-AgNPs were characterized using UV-visible spectroscopy and X-ray diffraction. Following this, MTT assay was done in vitro to evaluate the percent cell inhibition and half maximal inhibitory concentration (IC50). Among the various treatments, S-AgNPs were found to have higher percent cell inhibition as 77.61 percent. Trypan blue dye test was done to assess the percent of cell viability and number of viable cells. The cell viability in percentage for S-AgNPs was low (43.8 percent), whereas for C-AgNPs, it was 57.48 percent. After this, cells after treatment at IC50 concentration, were subjected for Acridine orange / Ethidium bromide staining. This revealed the manifestation of cells in the late apoptotic stage, treated with S-AgNPs. Hence, it may be concluded that silver nanoparticles biosynthesized from sesame seeds induces cytotoxicity in cancer cells.

Highlights

  • Silver nanoparticles from aqueous extract from seeds of Sesamum indicum and trisodium citrate were produced and characterized.
  • Dalton’s lymphoma ascitic cells were used for assessing anticancer activity.
  • Cytotoxic activity was analyzed using MTT, trypan blue and Acridine orange / Ethidium bromide staining.


© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited



Print This Article Email This Article to Your Friend

@ Journal of Animal Research | In Association with Association of Mastitis

36589936 - Visitors since March 23, 2019