Year: 2022 | Month: June | Volume 12 | Issue 3

Occurrence of Multidrug Resistant Coagulase negative Staphylococcus spp in Canine pyoderma, and Their Comparative Phenotype and Molecular Characterization

Naveena Antony Lakshmi Kavitha Kommalapati Vinod Kumar Nagaram Jagadeesh Babu Angalakuditi


A study was conducted on 137 clinical cases of canine pyoderma from July 2021 to November 2021. Out of 137 bacterial isolates, 129 (94.16%) isolates were Gram positive. Among them 125 were of Staphylococcus spp and accounted for 91.24% of total isolates. Among these 125 Staphylococcus isolates, 48 (38.4%) were identified as coagulase positive and 77 (61.6%) were identified as coagulase negative. Biochemically identified spp collections (S. lugdunensis - S40, S. simulans - S11, S. cohnii subsp. xylosus - S41 and S. cohnii subsp. urealyticus - S44) used for phylogenetic analysis of 16S rRNA gene nucleotide sequences. Sequence results identified S40 as S. saprophyticus, S11 as S. haemolyticus, S41, S44 as S. pseudintermedius and the sequences of the 16S rRNA was submitted to Genebank with accession numbers as OM302140, OM302142, OM302146, OM302147. The in vitro antibiotic sensitivity tests showed highest resistance to streptomycin (94, 75.2%) followed by cefoxitin (83, 66.4%). Most of the isolates were found to be susceptible to enrofloxacin (62.4%), amoxicillin + clavulanic acid (58.4%), and gentamicin (58.4%). Among the methicillin resistant strains, 37 were CPS isolates (S. aureus 9 and S. pseudintermedius 28) and 46 were CNS isolates. The distribution of methicillin resistant gene mecA was observed in 19 isolates (22.89%) of Staphylococcus. Out of these 19 isolates, four were methicillin resistant S. aureus (CPS), 14 were methicillin resistant S. pseudintermedius (CPS) and the remaining one was methicillin resistant CNS isolates. The results showed an increased pathogenesis and methicillin resistance of SIG group S. pseudintermedius in Canine pyoderma.


  • Biochemically identified spp collections used for phylogenetic analysis of 16S rRNA gene nucleotide sequences.
  • The methicillin resistance of SIG group S. pseudintermedius (mecA gene and pse gene) isolated from Canine pyoderma.

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