Year: 2023 | Month: October | Volume 13 | Issue 5

Enhancing Microbiota Analysis, Shelf-life, and Palatability Profile in Affordable Poultry Byproduct Pet Food Enriched with Diverse Fibers and Binders

Rishav Kumar Meena Goswami and Vikas Pathak


In this investigation, we examined the impact of incorporating fiber-rich vegetables and binders into pet food on microbiological aspects and storage stability under ambient conditions. Beyond achieving nutritional balance, assessing microbiological factors and storage durability is vital for ensuring the well-being of dogs. Varied levels of fiber-rich vegetables and binders were integrated into pet food formulations. Evaluation of pet food quality encompassed microbiological assessments, notably Coliform, Salmonella, yeast mold count, and total plate count, alongside physiochemical attributes like pH, TBARS, and FFA values. Pet food formulations containing poultry byproducts powder and a control group with chicken meat powder were stored at room temperature (25±1°C). Storage stability was assessed at 15-day intervals over 60 days. The highest mean pH values were observed in BP3>RG2=PO2>M3 treatments, while for TBARS, FFA values, and total plate count, the highest means were in BP3>M3>PO2>RG2. M3>BP3>PO2>RG2 exhibited the highest mean yeast and mold counts. Throughout storage, no Coliform or Salmonella counts were detected. PH, TBARS, FFA values, and microbiological counts significantly increased (P<0.05), while sensory attribute scores decreased (P<0.05) as storage progressed. RG2 demonstrated higher oxidation stability, lower microbiological counts, and significantly greater overall acceptability scores (P<0.05) compared to M3 and other treatments, lasting until the end of storage. The identified microorganism levels may have implications for prolonged consumption of contaminated food.


  • RG2 pet food excels in stability and oxidation resistance with varied fiber-rich ingredients during a 60-day storage.

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@ Journal of Animal Research | In Association with Association of Mastitis

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