Medicinal plants are worthy constituents of traditional medicine including therapy for the relief of pain. Evaluation of Antinociceptive

activity of Hygrophila spinosa hydro-alcoholic extract, cow urine distillate and their combination was done by observing latency in reaction time of mice to raise or lick the fore limb or jumping using Eddy’s hot plate analgesiometer. The reaction time recorded on Eddy’s hot plate analgesiometer for various treatment groups except control was highest at 60 min followed by 120 min, then 30 min and was least at 0 min. At 60 min, the reaction time showed by rats of Group II (Aspirin), Group III (0.5 ml CUD), Group IV (HSE: 400 mg/kg) and Group V (CUD: 0.5 ml + HSE: 400 mg/kg) were 14.833 ± 1.352, 10.833 ± 1.276, 13.000 ± 1.528 and 14.167 ± 0.946 seconds, respectively, which were significantly (p≤ 0.05) higher than the reaction time shown by control group rats (3.167 ± 0.477 seconds). The results endorsed the utility of Hygrophila spinosa in pain relief treatment as per Ayurvedic texts. The study has also proved the anti-nociceptive activity of cow urine distillate and its combination with H. spinosa hydro-alcoholic extract in mice.

Highlights

• Ash of Hygrophila is used with cow urine or water in inflammation.
• Pain inhibition by HSE in hot plate latency assay was comparable to Reference drug. CUD potentiated the analgesic action of HSE.