The present study was conducted to examine biochemical and microbiological properties of fresh cow urine. A total of 98 fresh urine samples from apparently healthy indigenous and crossbred cows maintained under organized farming system were collected aseptically in sterile vials. The urine samples were subjected to biochemical, microbial, yeast and mould examination using respective diagnostic kits and suitable culture media. Average fresh urine pH was significantly (p<0.05) higher in indigenous cows than in crossbred cows. No difference was observed for urea concentration between indigenous and crossbred cows. The average urea concentration was 1.56% in all the fresh urine samples collected from indigenous cows. Dry crossbred cows had significantly (p<0.01) higher urea concentration than in the milch cows, however, no difference was observed in milch and dry indigenous cows. Milch indigenous cows had significantly (p<0.05) lower creatinine concentration than in the dry cows. On microbial examination of different samples, bacterial growth was absent except four samples which showed bacterial colonies on BHI and MLA. The SDA method for fungal growth suggested no such growth in the study. The present study revealed that the fresh cow urine obtained from apparently healthy cows can be utilized for recommended preparations in agricultural operations.

Highlights

• The biochemical and microbiological characteristics of fresh urine collected from certain indigenous and crossbred cows were analyzed.
• Fresh cow urine free from microorganisms obtained from healthy cows can be utilized for recommended agricultural operations.