The field of genomics has been revolutionized by next generation sequencing. NGS technology is likely to play an important role in the field of veterinary medicine and animal husbandry. The feasibility of sequencing genomes at a much faster rate and with greater precision has been made possible with the advent of newer methods. In the current review, we describe the various sequencing methods available and also discuss select areas of biology where application of next generation sequencing would open a whole new avenue in veterinary research.

The present investigation was carried out on 459 Murrah buffaloes affected with some reproductive problem with 618 calving records(out of total 1336 Murrah buffaloes) sired by 118 sires over a period of 12 years from 2007 to 2018 at GADVASU, Ludhiana. The heritability estimates for dystocia, retention of placenta (ROP), anestrus, repeat breeding (RB), abortion and postpartum abnormal discharge (PPAD) were 0.04, 0.10, 0.05, 0.074, 0.085 and 0.121 respectively. The overall means for breeding values for dystocia, ROP, abortion, anestrus, RB and PPAD were 0.4, 0.2, 0.4, 0.2, 0.078 and 11.8%, respectively. Rank correlation of abortion with ROP was very high (0.99±0.02) and with anestrus, dystocia, RB and PPAD were also high i.e. 0.98±0.03, 0.98±0.03, 0.97±0.03, 0.95±0.04 respectively. Heritability estimates of reproductive problems have indicated appreciable values, signifying that incorporation of these traits in selection program will enhance the rate of improvement.

The present experiment was undertaken to study the relationship between clusterin (CLU) gene expression and in vitro sperm characteristics in buck semen. Fresh semen samples were collected from 12 bucks maintained in the organized goat farms by artificial vagina. Normalization of initial concentration of spermatozoa was carried out in all buck semen samples before proceeding for RNA isolation. So, initial concentration of each sample was made equal. The spermatozoa were isolated from buck semen samples by swim- up protocol using sperm TALP. Total RNA from the buck spermatozoa were extracted and first strand cDNA was synthesized from 1μg total RNA by using commercial kits. Absolute quantification of CLU gene transcripts in semen samples from 12 bulls was performed by plotting standard curve. Variations in levels of CLU gene transcripts (2500-22546000 copies) were found among 12 different buck semen samples. In vitro sperm characteristics were also studied from 12 buck semen samples. Variations in sperm characteristics such as sperm motility (60.0 - 80.0%), sperm viability (72.0 - 93.0%), sperm morphology (73.0 - 91.0%), plasma membrane integrity (50.0 - 82.0%), acrosome integrity (81.0 - 93.0%), DNA integrity (82.0 - 93.0%) and MMP (46.0 - 74.0%) were found among buck semen samples. All in vitro sperm characteristics were highly (negatively) correlated (p<0.01) with expression levels of CLU gene transcripts in spermatozoa. From this study, it is evident that ejaculated buck semen has variations in transcription pattern of CLU gene in spermatozoa among bucks and expression levels of CLU transcripts have negative correlation with in vitro sperm characteristics in buck semen samples.

Bacillus cereus incorporates the most important group of endospore-forming micro organism and can cause emetic and diarrheal food poisoning. A total of 42 B. cereus strains isolated from marketed raw chicken meat and human subjects swab samples were assessed by a triplex and multiplex PCR for the presence of enterotoxin genes. The detection rate of nheB, hblA, hblD, cytK, nheA,CER, hblC and entFM enterotoxin genes among all B. cereus strains was 83.33%, 80.95%, 69.04%, 21.42%, 47.61%, 0%, 61.90%, and 92.85% respectively. Enterotoxigenic profiles were determined in enterotoxin-producing strains showed 19 different patterns. The results offer essential information on toxin genes prevalence and toxigenic profiles of B. cereus from sources of origin. The present study was taken into consideration about extreme fitness danger for public health and insuring extra ability in difficulty to food safety amongst all B. cereus group members. Also, there may be need for extensive and continuous tracking of food products embracing both emetic toxin and enterotoxin genes.

Duck plague is an acute highly contagious disease of duck, geese and swan of all ages caused by Anatid Herpesvirus-1. The disease is characterized by significant decrease in growth, egg production and sudden death along with high morbidity and mortality which results into significant economic losses in duck industry. In present study, the pathology of wild strain (DP/As-Km/0019) of duck plague virus (DPV) was experimentally studied in 2 months old ducklings. The prominent clinical signs observed were depression, loss of appetite, greenish diarrhoea, ruffled feathers, nasal discharge, lacrimation and pasted eyelids with periorbital ring formation. The significant gross lesions were observed in spleen, oesophagus, liver, heart, brain and intestine. Vascular and degenerative changes like congestion, haemorrhages, necrosis and diphtheritic membrane formation were noticed in various parenchymatous organs. Microscopically, focal to diffuse areas of haemorrhages, coagulative necrosis, and fatty changes in liver, focal emphysema in lungs, formation of diphtheritic membrane on the mucosal surface along with presence of numerous infiltrating cells in oesophagus were recorded. Presence of Anatid Herpesvirus-1 viral DNA in liver and spleen tissue samples was detected by PCR.

Pregnancy toxaemia is a metabolic disorder that occurs in does and ewes during the late stage of pregnancy. Pregnant does that have low energy levels and having multiple numbers of fetuses are more susceptible to toxaemia. The present study was carried out in twenty five goats in advanced stage of pregnancy with the history of anorexia, torticolis, grinding of teeth, salivation and rigors. On clinical examination of animals, they were dull, depressed with tachycardia, tachypenia, opisthotonus and pale conjunctival mucous membrane. The biochemical parameters revealed hypoglycemia and hypocalcemia. Urine samples were collected and urine analysis revealed positive for ketone bodies. The goats were successfully treated with 25% dextrose i/v as a bolus, multiple electrolytes solution containing 5% dextrose i/v, glycerin orally and Vitamin B-complex injection intramuscularly and all the twenty five animals survived. Out of 25 animals medical termination of pregnancy was done in 21 cases and four animals delivered a live kid.

The present study evaluated the effect of circulatory trace mineral profiles on fertility response during estrus induction by progesterone based therapy in acyclic Sahiwal cattle. Sahiwal cows and post-pubertal heifers (n=25 each) with true anestrus condition (acyclicity confirmed ultrasonographically) were subjected to CIDR-based protocol (intravaginal CIDR application for 7 days, 500 IU eCG at CIDR removal, followed by estrus observation and AI). In heifers, additionally an injection of 5.0 mg estradiol valerate at CIDR insertion was given. Blood samples on days 0 (before CIDR application), 3, 5, 7 and 9 were collected. Almost similar estrus induction (68% vs. 64%) and subsequent conception rates (41.2% vs. 50%) during the first induced estrus were recorded in cows and heifers, respectively. During the course of treatment, significant (P<0.05) variation in plasma copper and zinc concentrations was recorded both in cows and heifers. Contrarily, manganese concentrations varied significantly (P<0.05) in cows only. However, iron profiles remained uniform during the course of treatment. Significantly (P<0.05) higher pre-treatment plasma concentrations of copper, zinc and manganese was recorded in those cows and heifers in which estrus was induced, compared to those in which estrus induction failed to occur. No significant difference (P>0.05) was recorded for iron concentration vis-à-vis estrus induction response. Hence, it is indicated that circulatory copper, zinc and manganese profiles influence the reproductives energy in acyclic animals and their suboptimal profiles may reduce the efficacy of estrus induction protocols in Sahiwal cattle.

Ovalbumin, a major protein of egg white plays many roles including providing nutrition to the developing embryo, acting as coagulating agent, folliculogenesis and angiogenesis in chicken and other animals. This protein is expressed mainly in magnum and then deposited over the yolk of the oocyte/zygote. Hence, it is important in formation of egg and is an essential target to measure. We cloned chicken ovalbumin CDS in pAcGFP-C1 vector and has been initially expressed in chicken primary magnum cell culture. The ovalbumin protein tagged with 6x Histidine was purified from cell culture and used for production of primary antibody in rat. The ovalbumin protein along with freund’s adjuvant was injected to the rat, booster was given, and finally, hyper-immune sera was collected from rat. The antisera was purified for isolation of IgG. The IgG was used as primary antibody for Western blotting. Through Western blotting, ovalbumin protein isolated from chicken magnum was detected and the protocol was established to detect chicken ovalbumin protein.

The aim of the research was to investigate the gross morphological, histological and histochemical structure of epididymis in local pig of Mizoram. The epididymis was collected from six apparently healthy adult Zovawk pigs. The epididymis appeared as a straight elongated tubular structure with reddish-white colour comprises of three regions; head, body and tail. The biometrical parameter including the weight, length and thickness (head, body and tail) were found to be higher in the left side of epididymis than the right side. Histologically, the duct of epididymis was surrounded by irregularly arranged tissue. The parenchyma of epididymis consists of tubules with distinct boundaries of connective tissues. The collagen and reticular fibers were abundant in tunica albuginea and connective tissue present between the tubules. However, few elastic fibers occurred in the capsule and only in the blood vessels in both the left and right epididymis of Zovawk pig. Histochemical studies revealed that basement membrane of tubules, blood vessels and stereocilia shows strong Periodic acid Schiff activity, whereas the basement membrane of tubules and stereocilia showed more concentration of acidic mucopolysaccharides. This is the first study to provide the detailed morphological, histological and histochemical structure of Zovawk epididymis.

The present study was planned to determine the effect of Epigallocatechin Gallate (EGCG), Alpha tocopherol and their combination as an antioxidant in TCM-199 media for in vitro maturation (IVM) and in vitro fertilization (IVF) in bovine oocytes. Cumulus-oocyte complexes (COCs) were aspirated from the ovaries derived from slaughter house and in vitro cultured in three groups using TCM-199 supplemented with EGCG @10 μM, Alpha tocopherol @100 μM, and Combination (EGCG @10 μM plus Alpha tocopherol @100 μM). Oocytes of a control group were matured in TCM-199 medium without any treatment. After IVM, cumulus-free oocytes were co-incubated with frozen-thawed spermatozoa for 15–18 h. Compared to no addition, the presence of EGCG @10 μM in medium during IVM significantly (p<0.05) increased the proportion of maturation and fertilization rate. Combination produced significantly higher percentage of in vitro matured bovine oocytes compared to the alpha tocopherol @100 μM alone. The results suggest that EGCG @10 μM in IVM medium had better effect than Alpha tocopherol alone and Combination on in vitro maturation and subsequent fertilization of bovine oocytes.

An experiment was conducted to assess the effect of inclusion level of different feed ingredients rich in omega-3 fatty acids tooptimizethe production of omega-3 enriched Japanese quail meat. The feeding trial was conducted in Japanese quail broiler chicks for five weeks (0 - 5 week) period. The experiment had six treatments (T1-PO 4%; T2 LO 4%: T3 LO 3% + SO 1%; T4 LO 2% + SO 2%; T5 LO 1% + SO 3%; T6 SO 4%) with various inclusion levels of different sources of omega-3 fatty acids (linseed oil / sardine oil) with six replicates in each treatment and each replicate had ten birds. Inclusion level of 3 % linseed oil and 1 % sardine fish oil was optimum for producing omega-3 fatty acids enriched Japanese quail meat. Japanese quail meat was enriched with ALA- 12.67%; EPA-2.11%; DHA-2.16% and n-3-16.95% (% of total fatty acids). Omega-3 fatty acids were enriched by 9.79 times and Omega-6 : Omega-3 ratio was narrowed by 9.8 times than that of control which is ideal for human consumption.

Fatty liver haemorrhagic syndrome (FLHS) in poultry is a metabolic disease. An outbreak of fatty liver haemorrhagic syndrome (FLHS) was detected by post mortem examination of broilers in a commercial farm. There was severe loss of production and sudden deaths with moderate mortality. Post mortem of the dead birds were performed and histopathological examination was done as per standard procedure. At necropsy, dead birds had pale combs and wattles with significant liver lesions. The liver was enlarged, friable and greasy, yellowish brown with firm fatty layer deposits and haematoma was noticed in abdominal cavity. Fat vacuoles were seen in liver sections which was also confirmed by special stain. Multiple factors like high dietary energy and stress of production may have precipitated the FLHS in broilers.

The present study was done with the objective of comparative efficacy of arteether and buparvaquone against theileriosis in cattle. Total 67 cattle suspected for theileriosis were screened on the basis of clinical and blood smear examination. Group I (n=6) was treated with Inj. buparvaquone @ 2.5 mg/kg body weight once and Group II (n=6) was treated with Inj. arteether @5 mg/kg body weight intramuscularly for three consecutive days. Rectal temperature, heart rate and respiration rate were significantly increased before treatment and in both the groups after treatment showed significant improvement. Haematological parameters showed significant decreased in Hb, PCV, TEC, TLC and neutrophil and significant increase in lymphocytes, monocytes and eosinophil. Non significant difference in basophil count was observed. After treatment, significant improvement was observed in mean hemoglobin, PCV, TEC, TLC, neutrophil, lymphocyte and eosinophil. Non significant improvement was observed in monocyte and basophil count. Present study revealed percent efficacy of arteether was 66.66% and buparvaquone 100%.

An investigation was carried out during the period from the month of April 2017 to March 2018 to study the non-infectious pathological conditions of liver and kidneys of pigs in and around Guwahati city. A total of 29 cases of non-infectious pathological condition of the liver and kidneys were recorded out of 53 necropsied pigs. The samples were collected for both histopathology as well as for bacterial isolation. Gross and histopathological studies revealed variable histopathological changes such as congestion, haemorrhage, fatty changes and necrosis (Centrilobular and panlobular) in the liver. Kidneys showed haemorrhages, nephrosis, fatty changes, necrosis, atrophy of glomeruli.

A prospective study was conducted to identify the risk factors associated with the incidence of Canine Parvoviral Enteritis (CPE) in dogs. Total of 120 animals screened using PCR assay, 72.50 percentage of animal were found positive for Canine Parvo Virus (CPV). Incidence in history of unvaccinated and vaccinated dogs was 79.69 and 64.29 per cent respectively. Age-wise predisposition of CPE indicated that the highest incidence was observed in both less than 3 months (78.08 %) and 3 to 6 months of age group (77.42 %) followed by 6 to 12 months of age group (34.50 %). Incidence of CPE in scheduled and unscheduled vaccination was 30.00 and 83.33 per cent respectively. In this study, unvaccinated status, unscheduled vaccination and young age groups are found to be significant risk factors associated with the occurrence of CPE.

Mycoplasma gallisepticum and Mycoplasma synoviae are worldwide in distribution including India and causes heavy economic losses to the poultry industry. The present study confirms the incidence of M. gallisepticum and M. synoviae infections by gross and microscopic pathology and PCR in broiler chickens. Incidence of pathogenic Mycoplasma spp. in broiler chickens with respiratory lesions was found to be 26.66%. Out of total 60 flocks, 6 flocks (10%) were found positive for M. gallisepticum and 12 (20%) were positive for M. synoviae. The incidence of M. synoviae was found to be higher than M. gallisepticum. Catarrhal tracheitis, focal pneumonic consolidation of lungs and airsacculitis were consistent lesions in M. gallisepticum and M. synoviae positive broiler chickens. However, synovitis/arthritis was not observed in any of the flock positive for M. synoviae. Incidence of E. coli amongst broiler chickens with respiratory lesions was found to be 40% by PCR targeting 16S rRNA gene and was higher than Mycoplasma spp. Mixed infection of M. gallisepticum and E. coli was detected in 3(9.09%) flocks, M. synoviae and E. coli in 4 (12.13%), M. gallisepticum and M. synoviae in 1(3.03%) and M. gallisepticum, M. synoviae and E. coli combined infection was detected in 1 (3.03%) flock. Mixed infection of M. gallisepticum and E. coli, M. synoviae and E. coli and M. gallisepticum, M. synoviae and E. coli revealed moderate to severe lesions of chronic complicated respiratory disease.

The present study was aimed on the genetic improvement of non-descriptive goats through estrus synchronization and by crossbreeding with Beetal goats they were reported to be a good milker, having higher body weight and dressing percentage. AVIKESIL-S, indigenously developed progesterone intra-vaginal sponges were utilized for estrus synchronization in does maintained at livestock farm complex, Veterinary College, Hassan. Response to estrus synchronization was noticed in all the does (100%) under study, irrespective of the genetic background. Natural service was practised with three mating designs ie. G1-Beetal × Beetal, G2-Beetal × non-descriptive, G-3 non-descriptive × non-descriptive. Pregnancy test was performed with ultrasonography at 45 days post-mating. The conception rates in does of G1, G2 and G3 groups were 87.5, 87.5, and 75.0% respectively. Kidding rate were 85.7, 85.7 and 100.0% in G1, G2 and G3 groups, respectively. Litter sizes were 1.5, 1 and 1 in G1, G2 and G3 groups, respectively. Average birth weight in G1, G2 and G3 groups were 3.82±0.20, 2.82±0.12 and 2.00±0.09 kg, respectively and average daily gain in G1, G2 and G3 groups were 42.86±1.69, 33.81±1.28, and 33.57±0.87 g, respectively. Better growth performances were noticed up to 3 months of study period in G1 and G2 compared to G3 groups. It could be concluded that combined utilization of ES technology and crossbreeding in non-descriptive goats would provide superior kids for bulk marketing and ease management by avoiding dispersed kidding. Also more number of animals would be available for selection thus increasing the selection intensity their by improving the genetic improvement.

Rumen collapse or rumen void is a syndrome usually observed in cattle affected with other primary diseases. It is a sequelae to other primary diseases due to total anorexia for several days. Recently purchased and calved animals brought into Large Animal Outpatient Medicine Unit, Madras Veterinary College Teaching Hospital, with the history of prolonged anorexia, mastitis and metritis were screened for rumen collapse. A total of 20 cases were included for the study. Animals were subjected to clinical, physical, rectal, haematological and biochemical examination. Anamnesis included were anorexia in all cases, high rise of temperature 15(75%) and high coloured urine 14(70%). An rumen collapsed in case of recently purchased animals were 12(60%). Clinical signs were anemia 18(90%), enlarged lymph node 17(85%), decreased rumen motility 17(85%), decreased or suspended rumination 16(80%). Rectal examination revealed a small sized rumen (rumen collapsed). Rumen fluid examination revealedvery few live protozoa or complete absence of protozoal motility and increased in MBRT (Methylene blue reduction test) time. The haematological examination revealed reduced level of Hb, PCV and RBC level. Biochemical examination revealed reduced level of total protein and other parameters were remain in the normal range. The suggestive of the primary diseases involved were hypocalcaemia, theileriosis, anaplasmosis, mastitis, septic metritis, indigestion and babesiosis. The animals were firstly treated for primary diseases followed by rumen cud transplantation and oral administration of rumenotorics bolus.

A feeding trial was conducted to discern the effect of dietary incorporation of aniseed and ginger rhizome powder on growth performance and nutrient utilization in commercial broiler chickens. A total of 120, day-old broiler chicks were divided randomly into 4 treatment groups with 3 replicates each i.e. 10 broiler chicks per replicate. The feeding trial lasted for 42 days viz., A metabolism trial was conducted during the 6th week of feeding trial to know the nutrient utilization. During the starter phase, maximum weight gain was recorded in broiler chicks of treatment group T3 (827.70 g) fed diet incorporated with 1.0% ginger rhizome powder followed by treatment groups T1, T2 and T4, however, there was no significant difference in body weight gain amongst the different treatment groups. During finisher phase, the average body weight gain was 1313.50, 1365.32, 1308.20 and 1291.36 g in broiler chicks of treatment groups T1, T2, T3 and T4, respectively and did not differ significantly among different groups. During entire feeding trial period (0-42 days), incorporation of aniseed and ginger rhizome powder in the basal diets non-significantly improved growth performance in terms of body weight gain, feed conversion ratio and performance index. The average cumulative body weight gain was 2120.57, 2152.75, 2135.90 and 2064.43 g in broiler chicks of treatment groups T1, T2, T3 and T4, respectively and did not differ significantly among different groups. There was no significant difference in nutrient utilization among different treatment groups of broiler chickens.

Bovine tuberculosis (bTB), a chronic infection in cattle caused by Mycobacterium tuberculosis/bovis, that impacts productivity and represents a major public health threat. Although the considerable economic costs and zoonotic risk consequences associated with the disease, accurate estimates of bTB prevalence are lacking in many countries, including India. Therefore, in the current study for collection of tubercular lesions the postmortem examination of 100 cattle was conducted. All major viscera and regional lymph nodes were examined and incised. Histopathology was performed in the cases where gross lesions were suggestive of tuberculosis. PCR was performed on the tissue and faecal samples by using IS6110 insertion sequence, Mycobacterium tuberculosis/bovis complex PCR kit. In 12 animals, nodular lesions with casseating mass suggestive of tuberculosis were observed in the lung tissue. All the 12 lung impression smear and only five faecal smear showed acid fast bacilli stained by Ziehl-Neelsen staining. Histologic features comprised a classic granuloma as a characteristic lesion of tuberculosis composed of a central caseous necrosis with mantle of macrophages, lymphocytes, plasma cells, epithelioid macrophages and Langhan’s giant cells and were observed in all 12 cases. All the tissue samples and 11 faecal samples were positive for the Mycobacterium tuberculosis complex using IS6110 sequence. 8 tissue samples and 4 faecal samples were positive by using Mycobacterium tuberculosis/bovis complex PCR kit. It can be concluded that there was good agreement between histopathology, acid fast staining and PCR. It can also be concluded that faecal samples which are easier to collect should be preferred for diagnosis of TB by PCR in cattle.

The aim of this experiment was to study the effect of different sugars and buffers combinations in the extenders viz. Tris citric acid fructose (TCF), Tris citric acid glucose (TCG), Sodium citrate fructose (SCF) and Sodium citrate glucose (SCG) on the quality of Cauda epididymal spermatozoa of ram during cryopreservation and post thaw. Spermatozoa were recovered from Cauda epididymidis by incision method. Samples showing ≥70 % progressive sperm motility were pooled. Each pooled cauda epididymal sperm sample was divided into four aliquots and spermatozoa in each aliquot were washed using isotonic buffer by double centrifugation. Washed spermatozoa in each aliquot were extended separately in the four different extenders using 20% egg yolk and 8% glycerol as cryoprotectant. The quality of spermatozoa was evaluated immediately after extension in the particular extenders (pre-freeze) and at post thaw. The percent sperm motility was significantly (p<0.05) higher for TCF (45.00±4.47) than TCG (27.50±6.55) and SCG (20.83±5.39) extenders at post thaw. The percentage of HOST reacted spermatozoa was significantly higher (P<0.05) for TCF (61.05±2.60) than SCF (45.81±4.90) and SCG (46.41±4.16) at post thaw. The percent intact acrosome was also significantly higher (P<0.05) in TCF (79.39±2.16), SCF (80.74±1.38) and SCG (78.34±2.94) than TCG (71.32±2.47) at post thaw. In conclusion, the use of fructose as energy source in the Tris extender (TCF) was found the best combination of buffer and sugar for maintaining higher sperm quality during cryopreservation of ram caudaepididymal spermatozoa.

The present research work was undertaken to assess the etiopathology and haematobiochemical alterations in anemic dogs of Anand region, Gujarat irrespective of their age, sex and breed. Total 78 dogs were screened for anemia and blood samples were collected from a 51 anemic dogs of Anand region of Gujarat to identify etiopathology and haematobiochemical parameters alterations. Statistical analysis, interpretation, different etiology and comparison of haematobiochemical alteration with scientific literature were carried out to understand pathophysiology of disease. Amongst 78 of 51 dogs, incidence of anemia was recoded 65.38 per cent in which highest was due to liver disorders (23.53%). Incidence of anemia was observed higher in in adult group (58.83%) as well as in male (52.94%). Most clinical variants of anemic dogs showed pale mucous membrane (86.27%), hypothermia (68.63%), tachycardia (70.59%), tachypnea (74.51%) and increased capillary refilling time (64.71%). Haematological indices of anemic dogs revealed Hb, TEC, PCV, MCH, MCHC and MCV decreased significantly (p<0.01), whereas TLC increased significantly (p<0.05) as compare to healthy dogs. Serum biochemistry revealed significantly (p<0.01) increased BUN, Creatinine, Glucose, AST and ALT levels as well as decreased in total protein level in anemic case as compare to healthy ones. Different etiological factors for developing in anemia in dogs as well as hematobiochemical alterations are mentioned, which will be useful for further detailed investigations and therapeutic management.

The present study was conducted to explore the effect of pregnancy, parity and stage of lactation on milk yield and milk composition in crossbred cattle maintained at Livestock Research Station, Thiruvazahmkunnu, Palakkad, under Kerala Veterinary and Animal Sciences University. The farm is located at latitude, longitude and altitude of 11o21’ N, 76o21’ E and 60-70 m above sea level and having a mean annual rainfall of 2,570 mm. Milk samples from 44 crossbred cows in different parity, stage of pregnancy and lactation were collected for seven consecutive days and composition was analyzed using Ekomilk Ultra pro milk analyzer (Milkana KAM 98-2A). The overall mean milk yield, fat, SNF, protein and lactose were 7.9 kg, 3.6%, 8.1%, 3.2% and 4.2%, respectively. Pregnant cows had significantly (p<0.05) lower milk yield (6.06 vs 8.30 kg) and significantly (p<0.05) higher lactose (4.35 vs 4.23%) and SNF per cent (8.28 vs 8.01%) when compared to non-pregnant cows. Order of parity didn’t have any influence on milk yield nor on the milk constituents. The mean milk yields during the three stages of lactation (<100 days, 100-200 days and >200 days) were 9.23, 7.57 and 6.52 kg/day, respectively, with peak yield being observed during the first stage of lactation (<100 days). The stage of lactation had significant effect (p<0.05) on milk yield and fat per cent whereas the other components were not influenced by the stage of lactation.