The present study was carried out to investigate occurrence, serovar distribution and clinicopathological attributes of leptospirosis among sheep from South Gujarat. A total of 41 blood and serum samples were randomly collected from apparently healthy and clinically ailing sheep of different breeds and age of either sex, reared in different fl ocks. Seropositivity was found to be 12.20% among sheep using the Microscopic Agglutination Test (MAT). Among clinically ailing and apparently healthy sheep, seropositivity was found to be 4.35% (1/23) and 22.22% (4/18), respectively with involvement of serovars Pomona, Canicola and Icterohaemorrhagiae. The hemato-biochemical and urinalyses results showed variation among seropositive and seronegative animals however, these differences were non-signifi cant. The study indicated seropositivity with serovar Pomona as main leptospiral serovar among sheep in South Gujarat, however, the specifi c conclusion on clinicopathological aspect could not be made. Though for prevention and control of the disease where an obvious alteration in the serovars causing the disease is common further epidemiological study is necessary.