Year: 2017 | Month: June | Volume 7 | Issue 3
Comparative Study of Three Different Media to Improve Semen Processing and Flow Cytometric Analysis in Bovine
Abstract:
The objective of this study was to compare the efficiency of three selected semen processing media: TRIS, TALP and HTF (Human Tubal Fluid) on sperm viability in post-preparation samples to improve flow cytometric analysis. Towards this aim, PI (Propidium Iodide) dye was used to evaluate sperm viability using flow cytometer. Two Cross breed (Karan Fries, KF) and Two Indian breed (Sahiwal, SW) bulls were used for different media optimization. The average sperm viability with TRIS, TALP & HTF media in cross breed (Karan fries) bulls was observed to be 92.09 ±1.93%, 82.61±2.71%, and 79.78±2.94% respectively, whereas, for Indian breeds (Sahiwal) it was 80.03±2.29%, 77.18±1.61 % and 71.32±2.51% respectively. The results revealed that viability of semen obtained from cross breed bulls (KF) is significantly higher in TRIS media (P<0.001) compared to TALP and HTF (P >0.05) whereas for Indian breed bulls (SW), viability of semen in TRIS and TALP is similar with no significant difference (P >0.05). When compared to HTF, semen of SW bull was found to have highest survivability in TRIS (80.03±2.29% vs 71.32±2.51% in TRIS and HTF respectively; P< 0.01). Hence, it can be concluded from the present study that overall TRIS medium is best suited for semen processing and flow cytometric analysis.
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