Year: 2017 | Month: October | Volume 7 | Issue 5
Effect of Ascorbic Acid on mRNA Expression of HSP70 Gene in WLH Egg Type Growers During Heat Stress
Abstract:
Meteorological factors such as high ambient temperature and high relative humidity exert adverse effects on poultry production. Heat stress results in poor growth performance, feed efficiency, egg production and higher mortality. The aim of the experiment was to explore and quantify the relative mRNA expression of HSP70 gene in relation to ascorbic acid supplementation in White Leghorn (WLH) egg type growers exposed to heat stress. A total of 96 WLH egg type growers of 10 weeks age, were randomly divided and maintained in controlled comfort (26±1.0°C) condition and heat stressed (40±5.0°C) conditions. Each group was divided into four subgroups with twelve birds each in two replicates for homogeneity of experimental design. G1 was designated to control group whereas, G2, G3 and G4 group was supplemented with 100, 200 and 300 mg ascorbic acid (AA) respectively. Relative expression analysis of HSP70 in liver tissue was done on day 42nd of the experiment using RT-PCR technique. The mRNA expression in egg type grower birds was significantly (p<0.01) down-regulated in all the treatment groups as compared to control group in both comfort and heat stressed condition. In comfort condition, maximum down-regulation (0.32 fold) was found in G3 group followed by G4 group (0.43 fold) as compared to control group. Similarly, in heat stressed condition, maximum down-regulation (0.31 fold) was found in G3 group, followed by G4 group (0.56 fold) in comparison to control group. The present investigation reveals that supplementation of ascorbic acid on the expression patterns of HSP70 gene provide an indication that AA may be useful in combating rigors of heat stress in chickens.
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