Year: 2017 | Month: October | Volume 7 | Issue 5
Comparison of Three Immunological Assays to Detect Infectious Bovine Rhinotracheitis (IBR) Antibodies in Buffaloes
Abstract:
The present study involved in analysis of 81 sera samples collected from Murrah (34), Surti (20) and non-descript buffaloes (27) with history of respiratory and reproductive disorders for IBR antibodies by indirect Enzyme linked immunosorbent assay (ELISA), Virus neutralization test (VNT) and Passive haemagglutination assay (PHA). The immunological assays viz., indirect ELISA, VNT and PHA revealed that 50.62%, 43.21 % and 27.16 % of IBR positivity respectively. The overall positivity irrespective of serological test was found to be 54.32 %. The sensitivity, specificity of PHA and VNT in comparison with ELISA were 46.34 %, 70.73 % and 85.00 %, 92.50 % respectively. Moderate agreement between ELISA and VNT (kappa = 0.56) and fair agreement between ELISA and PHA (kappa =0.38) were found for the detection of IBR antibodies. Significant difference (p < 0.01) was found between indirect ELISA and PHA in the detection of BHV-1 antibodies. VNT and PHA were found to be useful as the quantitative tests to estimate antibody level in buffalo whereas ELISA could be performed for herd screening programme.
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