Year: 2021 | Month: April | Volume 11 | Issue 2
Effect of Supplementation of Different Forms of Selenium on In Vitro Dry Matter Digestibility and Microbial Biomass Production
Nano selenium was synthesized by wet chemical method at laboratory level. In this study particle size, particle shape, zeta potential and selenium content were characterized by using particle size analyser (PSA) and inductively coupled plasma mass spectrometry (ICP-MS). The result revealed that selenium nano particle is spherical in shape with mean particle size of 31.8 ± 2.31 nano meter (nm) and concentration of selenium is 98.34 ± 2.8 per cent selenium that ensured the purity of nano selenium. The toxicity was analysed by MTT assay against Vero cell line. The nano selenium effectively inhibited the growth of Vero cells in a dose dependent manner. In vitro digestibility and microbial biomass production also evaluated on different form of selenium on basal diet at different levels. We used sodium selenite, selenocysteine and nano selenium. Based on the calculations, IC50 for nano selenium derived from selenium powder was 89.11 μg/ml. when selenium is added at graded level to the basal diet in any form resulted in significant increase (P<0.05) in digestibility parameter such as in vitro apparent dry matter digestibility, in vitro true dry matter digestibility and microbial biomass production at all levels of addition compared to when no selenium was added. From the results it can be inferred that spherical shaped, nano-selenium particles of size ranging 31.8 nm could be produced by wet chemical method at laboratory level.
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