Year: 2021 | Month: June | Volume 11 | Issue 3
Screening and Association of Polymorphism in Alpha-Lactalbumin Gene in Holstein Friesian Crossbred Cattle
This study was done to monitor milk protein variations in α-lactalbumin gene (LALBA) and to determine possible associations with various performance traits in Holstein Friesian (HF) crossbred cattle. Present study was conducted on 546 records of 60 HF crossbred cattle maintained at livestock farms, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab. The records consists of data pertaining to weight at first calving (WFC), first lactation milk yield (FLMY), first lactation 305-days milk yield (FL305-dMY), first lactation length (FLL), first peak yield (FPY), days to attain peak yield (DPY), age at first calving (AFC), first service period (FSP), first dry period (FDP) and first calving interval (FCI). PCR-RFLP technique was used to categorize the different alleles in the population. Genetic polymorphism was recognized by using MnlI restriction enzymatic digestion followed by agarose gel electrophoresis. The test revealed occurrence of two genetic variants “A” and “B” for LALBA gene which was further confirmed by performing sequencing of amplified fragments. Sequence alignment revealed 2 SNP’s in the whole 166 bp fragment, one SNP is present in the 5’UTR region of exon and one in promoter region. Gene regulation study revealed “Antp” transcription factor not binding at 35 to 44 bp due to point mutation at 37 bp of A>G, suggesting modulation of transcription. Association study showed significant (p≤0.05) relation of LALBA gene “BB” genotype with high first lactation peak yield (FLPY). Further investigations are required to elucidate the biological and practical relevance of these SNP’s and change in transcription factor.
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