Year: 2021 | Month: December | Volume 11 | Issue 6
Method Validation for Detection of Sulphamethazine Residue in Chicken Meat Samples by Simple Rapid Liquid Chromatography Technique
Choodamani Chandrakar
Sanjay Shakya
Anil Patyal
S.K. Tiwari
O.P. Mishra
V.K. Naik
Ankit Shukla
DOI:10.30954/2277-940X.06.2021.20
Abstract:
The occurrence of antibiotic residues in chicken meat constitutes a potential risk to the health of consumers. The present study describes the optimization and validation of a high-performance liquid chromatographic (HPLC) method for the determination of sulphamethazine (SMZ) in chicken meat using photo diode array detector (PDA) and C18 analytical column. The extraction method involving deproteinisation of the chicken sample followed by a solid phase extraction (SPE) clean-up of antibiotic residue has been optimized. The method was validated according to the European Commission Decision 2002/657/EC. The recoveries for the studied antibiotics ranged from 81.6–84% with relative standard deviations between 6.6 and 7.2%. The method was applied for the analysis of antibiotic residues in 16 raw chicken meat samples collected from Durg, Chhattisgarh, India. 3 samples found contaminated with antibiotic residues. The method has limit of quantification below the maximum residue limits (MRLs) and easy to perform, thus found suitable for performing routine analysis.
Highlights
- Study focused on the detection of sulphamethazine antibiotic residue in chicken meat samples.
- A rapid HPLC method with PDA detection was optimized for the detection of sulphamethazine residue.
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