Year: 2022 | Month: June | Volume 12 | Issue 3
In Vitro Evaluation of Antimicrobial Activity of Chitosan Against Mycobacterium smegmatis MTCC 994
Vimal Kumar
Harsh Sharma
Amit Kumar Singh
Shweta Sharma
DOI:10.30954/2277-940X.03.2022.9
Abstract:
Tuberculosis (TB) presently represent one of the biggest world health problems and hence it is urgent to find new drugs that allow better control of the outbreak and arrest the emergence of patients with multiple drug resistance tuberculosis (MDR-TB) and extensively drug resistant tuberculosis (XDR-TB) cases which have arisen alarmingly. There is a general consensus that antimicrobials from natural products might prove very effective and must be validated so that the same can be exploited for human well-being. The present study provides a scientific validity to chitosan, a natural and readily available compound as chitin over the crustacean body. The chitosan is biodegradable and biocompatible in nature and hence causes minimum hazards to the body. Chitosan solution have shown very effective antimicrobial property against M. smegmatis MTCC 994 which was selected for this study as this bacterium is non-pathogenic in nature, has fast growing rate and have similarity in cell wall composition with that of Mycobacterium tuberculosis. The disc diffusion assay as well as resazurin microtiter assay (REMA) methods were used to evaluate the antimicrobial activity of chitosan. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the chitosan solution were found around 117.18 μg/ml. This finding suggests that even at lower concentrations, chitosan is very effective and hence can be potent antimicrobial agent in future for Mycobacterium tuberculosis as the MDR and XDR TB are evolving rapidly. The current study recommends similar research against Mycobacterium tuberculosis to evaluate the potential of chitosan to be used as anti-tuberculosis agent.
Highlights
- We studied antimicrobial activity of chitosan against Mycobacterium smegmatis MTCC 994.
- The results are encouraging and chitosan can prove an efficient tool to fight TB in future for which further investigation is required.
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