Year: 2022 | Month: October | Volume 12 | Issue 5

Foldscope Based Detection of Rice Husk and Saw Dust Adulteration in Feed Ingredients and Thiram Contamination in Cereal Grains

Thadaveni Anitha Srungarapati Bindu Madhuri Govindaraj Alexander Amaravadhi Sarat Chandra Malisetty Venkateswarlu
DOI:10.30954/2277-940X.05.2022.6

Abstract:

A study was conducted to develop foldscope based methodologies to detect adulteration of rice husk and saw dust in feed ingredients and thiram contamination in cereal grains. Feed ingredients and adulterants were treated with standardized phloroglucinol-hydrochloric acid solution and evaluated based on appearance of pink colour in foldscope images on reaction with lignin. Thiram contaminated or uncontaminated maize and sorghum grains were treated with cuprous iodide based solutions. Pink colour was not observed on treatment with phloroglucinol-hydrochloric acid solution in the foldscope images of soyabean meal, rice bran, de-oiled rice bran and wheat bran while pink colour spots were noticed in groundnut cake, un-decorticated cottonseed cake, decorticated cottonseed cake, sunflower cake, rapeseed meal and red gram chuni. However, both rice husk and saw dust were completely in pink colour without any changes in their cellular structure in foldscope images. Starch granules of maize and sorghum showed white colour while the starch granules of thiram contaminated maize and sorghum were covered with light brown to amber colour on treatment with cuprous iodide in ammonia solution in foldscope images. Rice husk was most frequently found in de-oiled rice bran samples from various dairy farms under field conditions. It is concluded that phloroglucinol-hydrochloric acid solution and cuprous iodide in ammonia solution can be used as reagents to detect adulteration of rice husk and saw dust in feed ingredients and mixed feeds and thiram contamination in cereal grains, respectively on the spot under field conditions using foldscope.

Highlights

  • Foldscope can be used to detect adulteration of rice husk and saw dust in feed ingredients with phloroglucinol-hydrochloric acid solution.
  • Thiram contamination in cereal grains can be detected with cuprous iodide in ammonia solution using foldscope.


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