Year: 2022 | Month: October | Volume 12 | Issue 5

Comparative Evaluation of Lemon Grass and Orange Essential Oils as a Green Pesticide against House Flies Musca domestica in India

N. Rani G. Ponnudurai Aditi Kalita


The house fly Musca domestica is a cosmopolitan insect associated with vectoring of etiological agents. Pest management strategies for house flies are needed. Chemical control method commonly used against this pest, though effective, has some disadvantages, such as development of insect resistance and bioaccumulation. Presently bioinsecticides, especially those derived from plants have been increasingly evaluated in controlling insect of medical and veterinary importance. In order to search for effective and ecofriendly control agents, the essential oils of Sweet orange (Citrus sinensis) and lemon grass (Cymbopogon citrates) were evaluated larvicidal, pupicidal and adulticidal activity against house flies. The two essential oils were Gas chromatography / mass spectroscopy analysis of two oils showed that Citral (32.9 %), β-Citral (21.03 %), Eucalyptol (13.9%) and Limonene (7.09%) as main components of lemon grass. While Limonene (53.31%), Carvol (7.74%), Cis-carveol (5.54%) were the major constituents of Orange EO. The (LD)50 value for larvicidal activity of lemon grass and orange were 29.58 µl & 33.28 µl respectively. The (LD)50 value for pupicidal activity of the lemon grass was 18.2 µl while orange oil (LD)50 = 48.27 µl . In the adulticidal activity assay to determine the Eos of lemon grass and orange at the concentration of 200 µl took a minimum time of 4.3 ± 0.11 minute to cause 100 per cent fly mortality followed by orange oil is 10.5 ± 0.22 minutes. The result revealed that the essential oils of Lemon grass and orange oil have possessed larvicidal, Pupicidal and adulticide properties against Musca domestica.


  • Musca domestica is the most common pest in poultry farms in India.
  • House fly is known to transmit many pathogens.
  • Essential oils of Citrus sinensis and Cymbopogon citrates has larvicidal, pupicidal and adulticidal activity against house flies.

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