Year: 2022 | Month: October | Volume 12 | Issue 5

In Vitro Evaluation of Oocysticidal and Sporulation Inhibition Effects of Essential oil of Orange (Citrus sinensis) Against Eimeria tenella

Aditi Kalita G. Ponnudurai N. Rani G.A. Balasubramaniam P. Mekala


A study was carried out to assess the oocysticidal and sporulation inhibition effects of essential oil (Eos) of orange (Citrus sinensis) against oocysts of Eimeria tenella by in vitro assay. Essential oil was extracted from orange peel by hydro-distillation using Clevenger apparatus at 80º C for 1-2 hours. In the in vitro trials, 20, 30, and 40 per cent of working concentration, prepared from essential oil and ethanol mixture (1: 5 ratio stock solutions), were tested against freshly collected oocysts of Eimeria tenella. The results showed that 57.2 ± 0.14, 64.0 ± 0.18 and 42.2 ± 0.25 per cent of the sporulated oocysts and 6.8 ± 0.27, 19.3 ± 0.35 and 36.0 ± 0.33 per cent of unsporulated oocysts were found to have been damaged in the 20, 30 and 40 percent concentration of essential oil respectively. In the positive control group (1% Sodium hypochlorite), 78.0 ± 0.03 and 17.3 ± 0.21 per cent of sporulated and unsporulated oocysts, respectively were found damaged. While 81.31 ± 0.06 and 80.3 ± 0.03 per cent oocysts got sporulated and did not show any damage in the negative control groups (50 % Ethanol and water). The highest sporulation inhibition of 43.7 ± 0.15 per cent was recorded in the 40 per cent group.


  • Coccidiosis is one of the most common diseases worldwide and caused by Eimeria species.
  • Citrus based essential oil having oocysticidal and sporulation inhibition effect.
  • Essential oil of orange peel (30 %) can be used as organic disinfectant in broiler farms.

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