Year: 2022 | Month: August | 12 | 4
Hepatoprotective Effect of Pomegranate Juice Extract in Methotrexate Induced Hepatic Dysfunction in Rats
S. Pawankalyan
B. Kalakumar
P. Shivakumar
D. Madhuri
B. Anilkumar
Y. Ravikumar
DOI:10.30954/2277-940X.04.2022.3
Abstract:
The current study was aimed to assess the progression of hepatic fibrosis and to compare the therapeutic efficacy of pomegranate juice to the known anti-fibrotic drug, enalapril. In this study, a total of 24 rats were used and divided into 4 groups: groups I & II were kept as normal control & MTX controls, whilst groups III and IV were kept as MTX+ pomegranate and MTX+ enalapril, respectively, and the study was carried out for 28 days. Serum samples were collected on the 14th and 28th days for the estimation of AST, ALT, GGT, and TGF-1 levels. Rats were sacrificed at the end of the experiment, and liver samples were collected for further estimation of anti-oxidant levels and histopathology of the liver. The study results showed significantly elevated the activity of AST, ALT and GGT, TGF- β1 in MTX received rats group (II) compared to control group (I) along with elevated levels of TBARS and significant diminished antioxidant enzymes GSH, GPx, SOD and GST. However, group III receiving pomegranate juice revealed substantial improvement in all parameters and results were validated through the histopathology of the liver. The presence of phytoconstituents ellagic acid, gallic acid, anthocyanins and catechins, could abrogate MTX-induced hepatic fibrosis.
Highlights
- MTX causes changes in the liver due to release of free radicals.
- MTX is an anticancerous agents used to treat cancer, inflammation like rheumatoid arthritis.
- Pomegranate is a easily available in the market which has anti-inflammatory, antioxidant rich fruit and could able to ameliorates adverse effect caused by MTX.
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