Year: 2022 | Month: August | 12 | 4
Antibacterial and Antibiofilm Activities of Cinnamon Bark Oil and Thyme Oil Against Clinical Isolates of Multidrug Resistant Staphylococcus aureus
Sreeshma
T.S.
Suja Rani
S.
Uma
R.
Ambily
R.
DOI:10.30954/2277-940X.04.2022.4
Abstract:
The rising tides of multidrug resistant Staphylococcus aureus (MDRSA) infection necessitate the development of potential antibacterial and antibiofilm agents. Essential oils derived from medicinal plants are gaining popularity as prospective antibacterial agents in recent years. Hence, the current investigation was carried out to evaluate the antibacterial and antibiofilm properties of cinnamon bark oil (CNBO) and thyme oil (THMO) against MDRSA isolated from bovine mastitic milk. Eighteen isolates of Staphylococcus spp. were recovered from 30 bovine mastitic sample collected, among which six were identified as MDRSA organisms. Further, chemical composition of CNBO and THMO were determined by GC-MS analysis, which revealed the existence of several terpenoids. The antimicrobial susceptibility of these MDRSA isolates against CNBO and THMO was tested by disc diffusion and microbroth dilution tests which specified significant antimicrobial activity of CNBO and THMO as evident from their zone of bacterial growth inhibition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Subsequently, antibiofilm assays were performed using Congo red agar method and tissue culture plate-based crystal violet assays. The CNBO and THMO produced remarkable inhibition of biofilm formation and preformed biofilm, manifested as significantly reduced minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC). Moreover, CNBO was found to be more effective as compared to THMO against the MDRSA isolates. Thus, the present findings are indicative of the vital role of cinnamon bark oil and thyme oil in inhibiting the multidrug resistant S. aureus and associated biofilm formation.
Highlights
- Chemical composition of CNBO and THMO was assessed by GC-MS analysis.
- Disc diffusion, Minimum inhibitory concentration and Minimum bactericidal concentration were used for assessing antibacterial activity.
- Antibiofilm activity was measured using Congo red method and tissue culture plate-based crystal violet assay.
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