Year: 2022 | Month: August | 12 | 4

Molecular Characterization of Escherichia coli Isolated from Raw and Pasteurized Milk

Sandeep Kumar Surendra Singh Shekhawat Bincy Joseph D.S. Meena Subhash Chand Meena
DOI:10.30954/2277-940X.04.2022.7

Abstract:

In the present study, an attempt to isolate and identify E. coli from raw and pasteurized milk was made. A total of 120 samples comprising of raw milk (80) and pasteurized milk (40) were processed for the isolation of E. coli. These 120 samples of raw and pasteurized milk samples were collected randomly from local milk vendors, milk store , milk parlour, dairy booth and retail dairies located in different parts of Jaipur city, Rajasthan. Out of 120 samples, the prevalence of E. coli was recorded in raw milk and pasteurised milk as samples as 31.25% (25) and 27.5% (11) respectively. In the present investigation, 12 different antibiotics were used in to obtain antibiogram for 36 isolates of E. coli recovered from raw and pasteurized milk samples. The analysis of antibiogram revealed that the most effective antibiotic was Enrofloxacin (83.33%), followed by Nalidixic acid (75%) and Oxytetracycline (75%) of the isolates were sensitive. Also, 72.22% isolates were sensitive to Co-trimoxazole, 63.88% to Ceftriaxone and Trimethoprim respectively and other antibiotics were less effective. Erythromycin showed highest resistance (47.22%) followed by Trimethoprim (36.11%) and cefotaxime (30.55%). Out of 36 E. coli isolates, 17 (47.22%) were found to have MAR index more than 0.2, thus indicating injudicious use of antibiotics. On molecular profiling, all the 36 isolates were found to be positive for ITS gene, uidAgene and uspA gene. Out of the 36 isolates, one (2.77%) was positive for virulence gene (stx1).

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