Year: 2022 | Month: December | Volume 12 | Issue 6

Detection of Drug Resistance in Mycobacterium tuberculosis Complex Isolates From Cattle and Buffaloes

Poloju Deepa Gursimran Filia Deepti Narang Leishangthem Geeta Devi Sikh Tejinder Singh
DOI:10.30954/2277-940X.06.2022.24

Abstract:

Drug-resistant mycobacterial strains are increasingly being isolated from human cases. The identification of mycobacterial species in cattle and buffaloes is crucial for identifying the danger of drug-resistant strains being transmitted from animals to humans. In present study, a total of 200 animals (105 cattle and 95 buffaloes) from organized dairy farm were screened by CITT. Fifteen blood samples from animals tested by CITT, 57 tissue samples from dead animals and 6 trans-tracheal wash samples were collected from live animals and inoculated on Middlebrook 7H11 media and L-J media after proper decontamination. The isolates were identified by biochemical tests. DNA extracted from specimens and isolates was subjected to PCR for detection of MTC. The isolates positive for MTC were subjected to PCR for detection of drug resistance to rifampicin, isoniazid and streptomycin. Out of 57 tissue samples, 6 transtracheal washes and 15 blood samples, a total of 24 tissue samples, one transtracheal wash, and two blood samples were found to be positive by PCR respectively. Only 17 isolates were obtained from all of the samples isolated (16 from tissue and 1 from transtracheal wash), out of these, six isolates (5 tissues and 1 transtracheal washes) were confirmed as Mycobacterium bovis by biochemical tests and were found to be positive for MTC by PCR. Out of these 6 isolates, 4 isolates showed resistance to rifampicin. None of the isolates showed resistance to isoniazid and streptomycin.

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