Year: 2023 | Month: August | Volume 13 | Issue 4

In-silico Designing and Testing of Multiprotein Vaccine Construct Against Cow Milk Allergy

Nidhi Sukhija Kanaka K.K. Komal Jaglan Chethan Raj Sona Sukhija and Vidhyasagar


Cow’s milk allergy is a common diagnosis in infants and children, and it manifests as a hypersensitivity reaction to the allergenic
proteins in cow milk. Cow milk contains about 20 distinct protein components. The primary allergens are casein protein (alpha-s1-, alpha-s2-, beta-, and kappa-casein) and whey protein (alpha-lactalbumin and beta-lactoglobulin). There are several allergy treatments available, but they are just temporary. Developing a vaccine against milk allergy may appear utopian. Epitopes were found and exploited to build a multiprotein vaccine in this immunoinformatics study. Immunoinformatics techniques were used to predict T- and B-cell epitopes. Adjuvants were used to boost the vaccine’s antigenicity. The created vaccine design was shown to be soluble, antigenic, non-allergenic, and non-toxic.


  • Cow milk contains around 20 proteins which may cause allergy.
  • Immunoinformatics approach is used to design vaccine.
  • Allergens are identified and vaccine is constructed and tested in-silico.

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@ Journal of Animal Research | In Association with Association of Mastitis

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