Year: 2023 | Month: August | Volume 13 | Issue 4

In-silico Designing and Testing of Multiprotein Vaccine Construct Against Cow Milk Allergy

Nidhi Sukhija Kanaka K.K. Komal Jaglan Chethan Raj Sona Sukhija and Vidhyasagar
DOI:10.30954/2277-940X.04.2023.5

Abstract:

Cow’s milk allergy is a common diagnosis in infants and children, and it manifests as a hypersensitivity reaction to the allergenic
proteins in cow milk. Cow milk contains about 20 distinct protein components. The primary allergens are casein protein (alpha-s1-, alpha-s2-, beta-, and kappa-casein) and whey protein (alpha-lactalbumin and beta-lactoglobulin). There are several allergy treatments available, but they are just temporary. Developing a vaccine against milk allergy may appear utopian. Epitopes were found and exploited to build a multiprotein vaccine in this immunoinformatics study. Immunoinformatics techniques were used to predict T- and B-cell epitopes. Adjuvants were used to boost the vaccine’s antigenicity. The created vaccine design was shown to be soluble, antigenic, non-allergenic, and non-toxic.

Highlights

  • Cow milk contains around 20 proteins which may cause allergy.
  • Immunoinformatics approach is used to design vaccine.
  • Allergens are identified and vaccine is constructed and tested in-silico.


© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited



Print This Article Email This Article to Your Friend

@ Journal of Animal Research | In Association with Association of Mastitis

30305494 - Visitors since March 23, 2019