Year: 2025 | Month: April | Volume 15 | Issue 2
Determination of Virulence Factors and Antibiotic Resistance with Special Emphasis on Extended Spectrum β Lactamase Producing Escherichia coli from Sheep and Goats in Jammu, India
Shekhar Mishra
Mohd Rashid
Deep Shikha
Sabahat Gazal
Mehak Tikoo
Gulzar Badroo and Amitava Paul
DOI:10.30954/2277-940X.02.2025.5
Abstract:
The present study was aimed at investigating the pathogenic strains of Escherichia coli in sheep and goats and their antibiotic
resistance patterns with special emphasis on extended spectrum beta lactamases producing E. coli. A total of 200 presumptive
E. coli isolates were obtained from 120 faecal samples of sheep and goats. Out of these 200 isolates, 62 isolates showed the
presence of at least one virulence gene studied. Among these, 10 isolates carried eaeA gene either alone or in combination
with ehxA and were categorized as EPEC. 44 isolates carried stx genes and were categorized as STEC, 08 isolates carried ehxA
genes and were designated as EHEC. All 62 isolates showing the presence of at least one virulence gene were further screened
for ESBL production by using primers specific for blaTEM, blaCTX-M, blaSHV and blaOXA genes. Out of 62 isolates, only 22 were
tested positive for presence of ESBL genes. Further, the prevalence of blaTEM, blaCTX-M and blaSHV genes was found to be 54.54%,
27.27% and 36.36% respectively. Antibiotic sensitivity profiling of the isolates positive for virulence genes revealed that all
isolates were resistant to cefotaxime.
Highlights
- The most abundant ESBLs type in Sheep and Goat of Jammu region is blaTEM gene.
- Antibiotic sensitivity profiling shows highest resistance against cefotaxime.
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