The present experiment was conducted to evaluate the effect of vitamin-E-Selenium on stress parameters in anestrus Murrah buffaloes located at R.S. Pura, Jammu. Twelve animals with similar age and reproductive status were selected for experiment. The selected animals were divided in two groups, each comprising six animals. Group I was given used CIDR protocol while in group II animals along with used CIDR protocol, were administered two injections of vitamin-E-care-Se (50 mg α-tocopheryl acetate and 1.5 mg selenium per ml) at the dose rate of 1ml/50 kg b. wt. on day 0 and day 7 through intramuscular route. The blood samples were collected on day 0, day 7 and day 9 during the experiment. The collected samples were stored at -200C and later evaluated for oxidative stress parameters. A signifi cant decrease (P<0.05) in lipid peroxidation (LPO), superoxide dismutase (SOD) and catalase (CAT) activity along with non-signifi cant increase in glutathione peroxidase (GPx) level was observed when postpartum anestrus buffaloes were treated with used CIDR protocol either alone or in combination with vitamin-E and Selenium. The with used CIDR protocol in combination with vitamin-E and Selenium resulted in signifi cant reduction in oxidative stress parameters (except MDA) at day 7 and day 9. However, with respect to MDA, there was no signifi cant difference between two groups at start of treatment and after treatment.