The experiment was designed to evaluate the optimal concentration of egg yolk in extender for diluting Barbari buck semen during short-term storage at 4oC. A total of four Barbari bucks were used as semen donors during the experiment. The observed mean (±SE) valuesrecorded for percent live spermatozoa ranged from 63.5 ± 1.08 to 75.33 ± 0.99, percent progressive motile spermatozoa ranged from 54.17 ± 1.56 to 69.00 ± 0.82, percentage of HOST positive spermatozoa ranged from 60.83 ± 1.62 to 71.33 ± 1.54 while the acrosomal integrity ranged from 58.67 ± 1.563 to 71.33 ± 0.92 during the experiment. Three different patterns to evaluate capacitation like changes were observed under fl uorescence. The observed mean (±SE) values of pattern F ranged from 45.67 ± 1.54 to 62.33 ± 1.14, pattern B ranged from 22.00 ± 0.73 to 28.83 ± 1.58 while pattern AR represent capacitated spermatozoa ranged from 15.67 ± 0.71 to 25.00 ± 0.62 during the experiment. A significantly higher value (p<0.01) of different seminal attributes was recorded in samples diluted with 15% egg yolk followed by 10%, 20%, 5% and 2.5%. The results recorded during the experiment indicates that 15% egg yolk in the semen extender is best suited for semen dilution during chilling process in Barbaribuck.