Present study was carried out to know the seroprevalence of BHV-1 in the population of cattle and buffaloes from Chhattisgarh, India. A total of 464 serum samples were collected from cattle and buffaloes of different districts in Chhattisgarh. The collected serum samples were screened by Avidin-Biotin Enzyme Linked Immunosorbent Assay kit that recorded an overall seroprevalence of 34.69%. Out of 422 cattle serum samples, 158 (37.44%) were found positive compared to 3 (7.14%) serum samples out of 42 from buffaloes. In different age groups, there was variability in prevalence of BHV-1. Animals above 9 years of age showed the highest seropositivity (45.9%) whereas young animals between 0 to 2 years of age showed the minimum seropositivity (6.89%). Crossbred cattle showed higher seropositivity (40.42%) followed by non-descript cattle whereas indigenous cattle showed the seropositivity of 39.77% and 22.03%, respectively. Murrah, Nagpuri and indigenous buffaloes showed seropositivity of 0%, 3.03% and 50%, respectively. In the present study, seropositivity of 36.53% and 37.56% was recorded in male and female cattle, respectively. Male and female buffalo showed 11.11% and 6.06% seropositivity, respectively. Seropositivity of 45.45% was recorded in animals without clinical signs whereas animals with history of different clinical conditions showed 24.46% seropositivity. Rhinotracheitis, pustularvulvovaginitis, mastitis and balanoposthitis were the main clinical findings associated with the selected in research trial animals.